Por favor, use este identificador para citar o enlazar este ítem: http://www.dspace.uce.edu.ec/handle/25000/11552
Registro completo de metadatos
Campo DC Valor Lengua/Idioma
dc.contributor.authorUzcanga, Graciela L.-
dc.contributor.authorPérez Rojas, Yenis-
dc.contributor.authorCamargo, Rocío-
dc.contributor.authorIzquier, Adriana-
dc.contributor.authorNoda, José A.-
dc.contributor.authorChacín, Ronny-
dc.contributor.authorParra, Nereida-
dc.contributor.authorRon, Lenin-
dc.contributor.authorRodríguez Hidalgo, Richar-
dc.contributor.authorBubis, José-
dc.date.accessioned2017-08-05T19:28:37Z-
dc.date.available2017-08-05T19:28:37Z-
dc.date.issued2016-01-
dc.identifier.citationUzcanga, Graciela L. y otros (ene-2016). Serodiagnosis of bovine trypanosomosis caused by non-tsetse transmitted Serodiagnosis of Trypanosoma (Duttonella) vivax parasites using the soluble form of a Trypanozoon variant surface glycoprotein antigen. Revista Veterinary Parasitology, 218, pp. 31-42es_ES
dc.identifier.issn0304-4017-
dc.identifier.otherREPOSITORIO DIGITAL UCE-
dc.identifier.urihttp://www.dspace.uce.edu.ec/handle/25000/11552-
dc.description.abstractPrevious studies have shown that a 64-kDa antigen (p64) that was purified from the Venezuelan TeAp-N/D1 isolate of Trypanosoma (Trypanozoon) equiperdum corresponds to the soluble form of its predominant variant surface glycoprotein (VSG), and exhibited cross-reactivity with Trypanosoma (Duttonella) vivax. The course of experimental acute infections of bovines with T. vivax were followed by measuring whole anti-p64 antibodies and specific anti-p64 IgG and IgM antibodies in animal sera by indirect enzyme-linked immunosorbent assay (ELISA). The value of p64 to diagnose bovine trypanosomosis was also examined using 350 sera from healthy and T. vivax-infected cows living in a trypanosomosisendemic and enzootic stable area, and 48 sera obtained during a trypanosomosis outbreak. Serological assays showed that ∼70–80% of the infected sera contained anti-p64 antibodies, based on the comparative immunodetection of the T. equiperdum clarified antigenic fraction used as a reference test. In the absence of a gold standard, Bayesian analysis for multiple testing estimated a sensitivity and specificity of 71.6% and 98.8%, respectively, for the indirect ELISA using p64 as antigen. An apparent prevalence of 37.7% for bovine trypanosomosis infection was also estimated with a Bayesian approach when the p64 ELISA test was used. Employing blood from acute infected cows, the indirect ELISA response against p64 was contrasted with the microhematocrit centrifuge method and analyses by polymerase chain reaction (PCR) using specific primers targeting the inter-specific length variation of the internal transcribed spacer 1 region of the 18S ribosomal gene. The efficiency of p64 for the detection of anti-trypanosome antibodies in acute infected bovines was also corroborated serologically by comparing its response to that of the Indonesian Trypanosoma evansi Rode Trypanozoon antigen type (RoTat) 1.2 VSG, which possesses high specificity and sensitivity. As expected, PCR was the best method to detect parasites and diagnose bovine trypanosomosis; however, a substantial level of concordance (Cohen’s = 0.667) was obtained when serological tests using p64 and RoTat 1.2 VSG were compared. Additionally, an agglutination assay was designed using p64 covalently coupled to carboxylate-modified latex microparticles, which was proven here to be suitable for a fast qualitative diagnosis of bovine trypanosomosis.es_ES
dc.language.isoenges_ES
dc.publisherAmsterdam: Elsevieres_ES
dc.rightsopenAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/ec/es_ES
dc.subjectTRIPANOSOMOSIS BOVINOes_ES
dc.subjectTRIPANOSOMA VIVAXes_ES
dc.subjectVARIANTE GLICOPROTEÍNAS DE SUPERFICIEes_ES
dc.subjectINMUNOLÓGICA REACTIVIDAD CRUZADAes_ES
dc.subjectAGLUTINACIÓN EN LATEXMICROPARTICLESes_ES
dc.titleSerodiagnosis of bovine trypanosomosis caused by non-tsetse transmitted Serodiagnosis of Trypanosoma (Duttonella) vivax parasites using the soluble form of a Trypanozoon variant surface glycoprotein antigenes_ES
dc.typeArtículoes_ES
Aparece en las colecciones: Artículos Indexados

Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Serodiagnosis of bovine trypanosomosis caused by non-tsetsetransmitted Trypanosoma.pdfARTÍCULO A TEXTO COMPLETO1.17 MBAdobe PDFVisualizar/Abrir


Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.