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Título : Enzyme-linked immunosorbent assay for diagnosis of Amphimerus spp. liver fluke infection in humans
Autor : Cevallos, William
Calvopiña, Manuel
Nipáz, Victoria
Vicente Santiago, Belén
López Albán, Julio
Fernández Soto, Pedro
Guevara, Ángel
Muro, Antonio
Palabras clave : AMPHIMERUS SPP
DIAGNÓSTICO
ENFERMEDAD DEL HÍGADO
Fecha de publicación : 2017
Editorial : Río de Janeiro: Ibsal
Citación : Cevallos, William y otros (2017). Enzyme-linked immunosorbent assay for diagnosis of Amphimerus spp. liver fluke infection in humans. Revista Memórias do Instituto Oswaldo Cruz, 112(105), pp. 1-6
Resumen : BACKGROUND Amphimerus spp. is a liver fluke that infects humans and domestic animals. It is highly prevalent in some Ecuadorian communities. Currently, diagnosis is based on the microscopic observation of eggs in faeces, but this has variable sensitivity. More sensitive methods are needed for diagnostic testing. OBJECTIVE The main objective of this work was to develop an enzyme-linked immunosorbent assay (ELISA) using crude antigens from Amphimerus spp. adult worms to detect anti-Amphimerus IgG in human sera. METHODS Crude somatic antigens were obtained from adult Amphimerus spp. worms. Human sera from 119 patients were tested: 48 from individuals with a confirmed Amphimerus spp. infection, 78 from non-infected Ecuadorians living in the endemic region, 60 from persons living in non-endemic areas (20 Ecuadorians, 20 Europeans, and 20 Africans), and 33 who had other parasitic and non-parasitic infections. PRINCIPAL FINDINGS Results were analysed using the receiver-operator characteristic (ROC) curve analysis with an area under curve (AUC) value of 0.967. The accuracy of the ELISA was high. The sensitivity was 85.0% [95% confidence interval (CI): 80.3- 89.7%] and the specificity was 71.0% (95% CI: 65.2-76.8%). Some cross reactivity was detected against Paragonimus mexicanus, Fasciola hepatica, Schistosomiasis, Taenia solium, Strongyloides stercoralis, Mansonella spp., and Vampirolepis nana. MAIN CONCLUSIONS We have developed the first ELISA technique that detects anti-Amphimerus IgG in human sera with good sensitivity, repeatability and reproducibility. However, more specific antigens are needed to further enhance performance of this assay. Regardless, this ELISA test could be useful for early diagnosis and prompt treatment of human Amphimerus spp. infections
URI : http://www.dspace.uce.edu.ec/handle/25000/13680
ISSN : 1678-8060
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