Por favor, use este identificador para citar o enlazar este ítem: http://www.dspace.uce.edu.ec/handle/25000/14477
Título : A new DNA extraction protocol for screwworm fly Cochliomyia species (Diptera: Calliphoridae)
Autor : Echeverría Fonseca, Gustavo
Mera, Ruiz Patricia
Carrillo Toro, Jenny Soraya
Rodriguez Hidalgo, Richar
Palabras clave : COCHLIOMYIA SPP
Fecha de publicación : ene-2015
Editorial : Suiza: Frontiers in Environmental Science
Citación : Rodriguez Hidalgo, Richar y otros (2015). A new DNA extraction protocol for screwworm fly Cochliomyia species (Diptera: Calliphoridae). Frontiers in Environmental Science, 2. 1-5.
Resumen : Modifications to the DNA isolation protocol from Cochliomyia spp., are suggested based on the Chelex® 100 reactive. To apply the sterile insect technique (SIT) program it is necessary to study the molecular variations of endemic populations with efficient, fast and low costs techniques. The test samples were collected in the Pichincha province of Ecuador. The isolation protocol had 3 steps: (a) pretreatment (optional), (b) mechanic and chemical lysis, (c) two incubations; then the supernatant were separated by centrifugation. Furthermore, variations in concentrations of magnesium chloride present in the master mix were evaluated. Results showed a high efficiency in isolation with approximately 1.20 h of manipulation (without pretreatment). Additionally, the quality of the amplicon that was visualized on 2% agarose (w/v) showed that the magnesium chloride concentration was influential in the PCR reaction mix.
URI : http://www.dspace.uce.edu.ec/handle/25000/14477
Aparece en las colecciones: Artículos Indexados

Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
A new DNA extraction protocol for screwworm fly Cochliomyia species Diptera Calliphoridae.pdfARTICULO A TEXTO COMPLETO709.14 kBAdobe PDFVisualizar/Abrir

Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.