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Título : Comparison of conventional techniques to differentiate between taenia solium and taenia saginata and an improved polymerase chain reaction-restriction fragment length polymorphism assay using a mitochondrial 12s RDNA fragment
Autor : Rodriguez Hidalgo, R
Benítez Ortiz, Washington Vicente
Geerts, S.
Geysen, Dirk
Brandt, Jef
Palabras clave : PROGLÓTIDES
ESTUDIOS MORFOLÓGICOS
TAENIA SAGINATA
Fecha de publicación : 2002
Editorial : Kansas: American Society of Parasitologists
Citación : Benítez Ortíz, Washington y otros (2002). Comparison of conventional techniques to differentiate between taenia solium and taenia saginata and an improved polymerase chain reaction-restriction fragment length polymorphism assay using a mitochondrial 12s RDNA fragment. BioOne Research Evolved, 88(5): 1007-1011
Resumen : Given the constraints of classical diagnostic methods, i.e., morphological and isoenzymatic studies of proglottids, a polymerase chain reaction test complemented with restriction enzyme analysis has been modified by redesigning one of the primers to reduce nonspecific amplifications experienced when using field samples. The use of these new, highly cestode-specific primers and the restriction enzyme DdeI led to the development of a diagnostic assay that clearly distinguishes between Taenia saginata and T. solium proglottids in field samples. This assay confirms the presence of T. saginata in Ecuador. DNA amplification of some of these taeniids showed different patterns, suggesting the possibility that strain differences exist. These results demonstrate the need for development of useful molecular assays as reliable tools for epidemiological studies on cestodes.
URI : http://www.dspace.uce.edu.ec/handle/25000/14633
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