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Título : Use of Real-Time Polymerase Chain Reaction to Differentiate between Pathogenic Entamoeba histolytica and the Nonpathogenic Entamoeba dispar in Ecuador
Autor : Guevara, Angel
Vicuña, Yosselin
Costales, Denisse
Vivero, Sandra
Anselmi, Mariella
Bisoffi, Zeno
Formenti, Fabio
Palabras clave : PARÁSITOS INTESTINALES
MUESTRAS DE LABORATORIO
POLIMERASA
ENTAMOEBA HISTOLYTICA
Fecha de publicación : 2018
Editorial : [s.d.t.]
Citación : Guevara, Angel y otros (2018). Use of Real-Time Polymerase Chain Reaction to Differentiate between Pathogenic Entamoeba histolytica and the Nonpathogenic Entamoeba dispar in Ecuador. The American Society of Tropical Medicine and Hygiene: 1-2
Resumen : Microscopic examination of stool samples has been considered to be the “gold standard” for diagnosis of intestinal parasites. Recently, polymerase chain reaction (PCR) has been approved by the World Health Organization as the method of choice for the diagnosis of Entamoeba histolytica. Of the 106 stool samples collected from the Esmeraldas and Pichincha provinces of Ecuador, all (100%) were positive for E. histolytica/Entamoeba dispar by light microscopy, whereas using real-time PCR (RT-PCR) DNA amplification, 74 (69.8%) were positive for E. dispar and only three (2.8%) were positive for E. histolytica. Some 29 (27.4%) samples were negative for the presence of either E. histolytica or E. dispar, this may be due the presence of Entamoeba mosksvskii, which is morphologically identical to E. histolytica/ E. dispar and not specifically targeted by the RT-PCR used. These results indicate the necessity of reevaluating the epidemiology of amebiasis in Ecuador as the prominent species found are nonpathogenic.
URI : http://www.dspace.uce.edu.ec/handle/25000/16836
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