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Título : A rapid molecular diagnosis of cutaneous leishmaniasis bycolorimetric malachite green-loop-mediated isothermal amplification(LAMP) combined with an FTA card as a direct sampling tool
Autor : Nzelu, Chukwunonso O.
Cáceres, Abraham G.
Guerrero-Quincho, Silvia
Tineo-Villafuerte, Edwin
Rodriquez-Delfin, Luis
Mimori, Tatsuyuki
Uezato, Hiroshi
Katakura, Ken
Gomez, Eduardo A.
Guevara, Angel G.
Hashiguchi, Yoshihisa
Kato, Hirotomo
Palabras clave : LEISHMANIASIS
INCUBACIÓN
CALORIMETRÍA
Fecha de publicación : 26-oct-2015
Editorial : México: Elsevier
Citación : Nzelu, Chukwunonso O. y otros (octubre 2015). Rapid molecular diagnosis of cutaneous leishmaniasis bycolorimetric malachite green-loop-mediated isothermal amplification(LAMP) combined with an FTA card as a direct sampling tool. Acta Trópica, 153, pp. 116-119
Resumen : Leishmaniasis remains one of the world’s most neglected diseases, and early detection of the infectiousagent, especially in developing countries, will require a simple and rapid test. In this study, we establisheda quick, one-step, single-tube, highly sensitive loop-mediated isothermal amplification (LAMP) assay forrapid detection of Leishmania DNA from tissue materials spotted on an FTA card. An FTA-LAMP withpre-added malachite green was performed at 64◦C for 60 min using a heating block and/or water bathand DNA amplification was detected immediately after incubation. The LAMP assay had high detectionsensitivity down to a level of 0.01 parasites per l. The field- and clinic-applicability of the colorimetricFTA-LAMP assay was demonstrated with 122 clinical samples collected from patients suspected of havingcutaneous leishmaniasis in Peru, from which 71 positives were detected. The LAMP assay in combinationwith an FTA card described here is rapid and sensitive, as well as simple to perform, and has great potentialusefulness for diagnosis and surveillance of leishmaniasis in endemic areas.
URI : http://www.dspace.uce.edu.ec/handle/25000/5181
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