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Título : Comparison of S. stercoralis serology performed on dried blood spots and on conventional serum samples
Autor : Formenti, Fabio
Buonfrate, Dora
Prandi, Rosanna
Marquez, Monica
Caicedo, Cynthia
Rizzi, Eleonora
Guevara Espinoza, Ángel Gustavo
Vicuña, Yosselin
Huerlo, Francisco R.
Perandin, Francesca
Bisoffi, Zeno
Anselmi, Mariella
Palabras clave : SEROLOGÍA
ENFERMEDADES INFECCIOSAS
STERCORALIS
Fecha de publicación : 24-oct-2016
Editorial : Suiza: Frontiers Media
Citación : Formenti, Favio y otros (2016). Comparison of S. stercoralis serology performed on dried blood spots and on conventional serum samples. Journal Frontiers in Microbiology, 7(1778), pp. 1-14
Resumen : Background. Dried blood spots (DBS) are used for epidemiological surveys on infectious diseases in settings where limited resources are available. In fact, DBS can help to overcome logistic difficulties for the collection, transport and storage of biological specimens. Objective. To evaluate the accuracy of S. stercoralis serology performed on DBS. Methods. A survey was proposed to children attending a school in the village of Borbon, Ecuador, and to their parents/guardians. Each participant gave consent to the collection of both serum and DBS specimens. DBS absorbed on filter papers were analyzed with a commercially-available ELISA test for Strongyloides stercoralis antibodies, as well as with standard serology. The agreement between the two methods was assessed through the Cohen’s kappa coefficient. Results. The study sample was composed of 174 children and 61 adults, for a total of 235 serum and 235 DBS samples. The serology was positive in 31/235 (13%) serum samples, and in 27/235 (11%) DBS: 4 samples resulted discordant (positive at 31 – 235 (13%).
URI : http://www.dspace.uce.edu.ec/handle/25000/7970
ISSN : 1664-302X
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